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KMID : 0360220080490101665
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Journal of the Korean Ophthalmological Society
2008 Volume.49 No. 10 p.1665 ~ p.1670
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Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells
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Kim Jae-Woo
Kim Sin-Hoo
Lee Jae-Hyung
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Abstract
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Purpose: To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence.
Methods: Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-β-gal assays, respectively.
Results: Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence.
Conclusions: Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.
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KEYWORD
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Ascorbic acid, Hydrogen peroxide, Oxidative stress, Senescence, Trabecular meshwork cells
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